Modern genetic evidence now points at a single domestication event for a limited number of
stallions, combined with repeated restocking of wild
mares into domesticated herds. This suggests that different body types might be a combination of both selective breeding and
semi-feral landrace traits. A study in 2012 that performed
genomic sampling on 300 work horses from local areas as well as a review of previous studies of archaeology,
mitochondrial DNA, and
Y-DNA suggested that horses were originally domesticated in the western part of the Eurasian steppe. Both domesticated stallions and mares spread out from this area, and then some additional wild mares were added from local herds; wild mares were easier to handle than wild stallions. Most other parts of the world were ruled out as sites for horse domestication, either due to climate unsuitable for an indigenous wild horse population or no evidence of domestication. It remains possible that a second, independent, domestication site might exist in the
Iberian Peninsula, but the study could neither confirm nor disprove that
hypothesis. The
Przewalski's horse (now
Equus ferus przewalskii) is currently believed to be unrelated to the modern domestic horse, though studies using DNA have had varied results. Recent mitochondrial DNA analysis suggests that the Przewalski and the modern domestic horse diverged some 160,000 years ago. Studies using DNA have been inconclusive. A 2009 molecular study using
ancient DNA (that is DNA recovered from archaeological finds like bones and teeth) places the Przewalski's horse in the middle of the domesticated horses. These difficulties exist in part due to crossing domestic horses into the Przewalski's horse as well as the limited genetic variation present in the
founder population of the modern Przewalski's horse.
Chariot burials about 2500 BCE present the most direct hard evidence of horses used as
working animals. Indirect evidence suggests that horses were ridden long before they were driven, approximately 3500 BCE. One theory proposed was that the modern horse is descended from the
Botai culture (in present-day Kazakhstan) where horses were milked and possibly
ridden more than 5,000 years ago. A study of ancient and modern horse DNA concluded that modern horses do share a small amount of DNA with Botai horses but modern horses are not descendants of Botai horses. Przewalski's horse and Botai horses were both descendants of another domesticated horse they called the Borly4.