The distinctive Lithuanian letter
Ė was used for the first time in the Klein's
Grammatica Litvanica and firmly established itself in the Lithuanian language; it is still in use today. In the
Grammatica Litvanica Klein also established the use of the letter for marking the sound
[v], the use of which was later abolished in the Lithuanian language in favor of . The
Grammatica Litvanica and other grammars written by Klein had great significance for the development of Lithuanian
linguistics as they systematically described the grammatical structure of the Lithuanian language for the first time, and established more stable general language norms, based on the
dialect of Western Aukštaitians (). ==An unpublished Lithuanian grammar from the 1640s and the different methods of the two authors==