In the
viral families
Paramyxoviridae and
Orthomyxoviridae, viruses use a homotrimeric
glycoprotein hemagglutinin on their
protein capsids. Hemagglutinins are responsible for binding to
receptors,
sialic acid residues, on host cell membranes to initiate virus docking and
infection. Specifically, they recognize
cell-surface glycoconjugates containing sialic acid on the surface of host
red blood cells with a low affinity and use them to enter the
endosome of host cells. Hemagglutinins tend to recognize α-2,6-linked sialic acids of the host cells in humans and α-2,3-linked sialic acids in avian species, although there is evidence that hemagglutinin specificity can vary. This correlates to the fact that
Influenza A typically establishes infections in the upper respiratory tract in humans, where many of these α-2,6-linked sialic acids are present. There are various subtypes of hemagglutinins, in which H1, H2, and H3 are known to have human susceptibility. It is the variation in hemagglutinin (and
neuraminidase) subtypes that require health organizations (ex.
WHO) to constantly update and surveil the known circulating flu viruses in human and animal populations (ex.
H5N1). In the endosome, hemagglutinins undergo conformational changes due to a
pH drop to of 5–6.5 enabling viral attachment through a fusion
peptide.
Types •
Influenza hemagglutinin: a
homotrimeric
glycoprotein that is found on the surface of
influenza viruses which is responsible for their infectivity. Influenza strains are named for the specific hemagglutinin variant they produce, along with the specific variant of another surface protein,
neuraminidase. • These hemagglutinins are subject to rapid evolution via
antigenic shift and
drift in the influenza avian reservoir. This results in new subtype of hemagglutinins being created frequently, and is the cause of seasonal influenza outbreaks in humans. •
Measles hemagglutinin: a hemagglutinin produced by the
measles virus that
encodes six
structural proteins, with hemagglutinin and fusion proteins being surface glycoproteins involved in attachment and entry. •
Parainfluenza hemagglutinin-neuraminidase: a type of
hemagglutinin-neuraminidase produced by
parainfluenza, which is closely associated with both human and veterinary disease. •
Mumps hemagglutinin-neuraminidase: a kind of hemagglutinin that the
mumps virus (MuV) produces.
Structure Hemagglutinins are small proteins that extend from the surface of the virus membrane as spikes that are 135 Angstroms (Å) in length and 30-50 Å in diameter. Each spike is composed of three identical
monomer subunits, making the protein a
homotrimer. These monomers are formed of two
glycopeptides, HA1 and HA2, and linked by two
disulphide polypeptides, including membrane-distal HA1 and the smaller membrane-proximal HA2.
X-ray crystallography,
NMR spectroscopy, and
cryo-electron microscopy were used to solve the protein's structure, the majority of which is
α-helical. In addition to the homotrimeric core structure, hemagglutinins have four subdomains: the membrane-distal receptor binding R subdomain, the vestigial domain E, that functions as a receptor-destroying
esterase, the fusion domain F, and the membrane anchor subdomain M. The membrane anchor subdomain forms elastic protein chains linking the hemagglutinin to the
ectodomain.
Mechanism On the viral capsids of influenza types
A and
B, hemagglutinin is initially inactive. Only when cleaved by host proteins, does each monomer polypeptide of the homotrimer transforms into a dimer – composed of HA1 and HA2 subunits attached by disulfide bridges. The HA1 subunit is responsible for docking the viral capsid onto the host cell by binding to sialic acid residues present on the surface of host respiratory cells. This binding triggers
endocytosis. The pH in the endosomal compartment then decreases from proton influx, and this causes a conformational change in HA that forces the HA2 subunit to "flip outward." The HA2 subunit is responsible for membrane fusion. It binds to the endosomal membrane, pulling the viral capsid membrane and the endosomal membrane tightly together, eventually forming a pore through which the viral genome can enter into the host cell cytoplasm. From here, the virus can use host machinery to proliferate. == Plants ==