He married Anne Gill, daughter of Benjamin Gill, Gentleman, and Mary Mainwaring (a descendant of
Thomas Stanley, King of Mann). Anne Gill served as a
lady-in-waiting to
Queen Henrietta Maria, wife of
King Charles I. Their children included: • Henrietta Marie (named after
Queen Henrietta Maria) • James Neale II (he married Elizabeth Lord, daughter of Captain John Lord who was a magistrate in Westmoreland County, Virginia) • Dorothy Neale • Anthony Neale • Jane Neale ==Legacy==