After her semester at Vassar, Tutwiler accepted a faculty position at Greensboro Female Academy,
Greensboro, Alabama, in the autumn of 1866. The following year, she was chosen to be the Academy's principala position she held for two years. Tutwiler served with her uncle as co-president of Livingston State Normal School. She was the first (and only) female president of the college. After decades of expansion, it became the University of West Alabama. With her support, in 1892 ten Livingston-educated students became the first women admitted to the
University of Alabama. She was called the "mother of co-education in Alabama". She was a key figure in the creation of the Alabama Girls' Industrial School, in October 1896. This institution eventually evolved into the
University of Montevallo. ==Prison reform==