. From left to right:- Negative control-
M. tuberculosis: Dry-appearing colonies-
Mycobacterium avium complex: Wet-appearing colonies-
M. gordonae: Yellowish colonies
Gram-positive, nonmotile and moderate to long
acid-fast rods. • Commonly found in tap water and soil. Casual resident in human
sputum and
gastric lavage specimens.
Colony characteristics • Smooth, with yellow or orange
scotochromogenic colonies. Even though they are
scotochromogenic pigment is intensified by growing in continuous light.
Physiology • Growth on
Löwenstein-Jensen medium and
Middlebrook 7H10 agar within 7 or more days at 37 °C (optimal 25 °C). • Does not grow in the presence of ethambutol (1 mg/L), isoniazid (10 mg/L) and sodium chloride (5%). • Some strains can grow using carbon monoxide as a carbon and energy source. Differential characteristics • A commercial
hybridisation assay (
AccuProbe) to identify M. gordonae exists. • Intraspecies variability in
16S rDNA sequences ==Pathogenesis==