On 21 September 1939 the
Kriegsmarine requisitioned
Donau, and ordered her to Hamburg to serve as a
troop ship to
East Prussia. Only nine of the prisoners survived the Second World War. On 19 December 1942
Donau ran aground in
Oslofjord. On 24 October 1943 she left
Turku in Finland for Oslo. In January 1944 she was again at the disposal of the head of maritime transport in occupied Norway. From 20 to 23 February she sailed from Oslo to Tallinn. That July she was in the
Skagerrak, and in August and October she was in the
Baltic. In November 1944
Donau sailed in a convoy to Norway.
Allied aircraft bombed the convoy in the Skagerrak on 26 and 27 November, but the convoy returned fire, and
Donau and her sister ship
Isar safely reached their destination in
Langesund. They made their return voyage in the first week of December. , who with
Roy Nielsen limpet mined
Donau in 1945, visiting her wreck after it was raised in 1952 In January 1945
Donau shuttled between
Aarhus in Denmark and Oslo in Norway. On or shortly before 16 January,
Roy Nielsen of
Milorg and
Max Manus of
Kompani Linge planted ten
limpet mines below the waterline along a section of the
port side of
Donaus hull, while she was docked in Oslo. The mines were meant to detonate in open sea once the ship had left Oslofjord, but her departure on the morning of 17 January 1945 was delayed, so they detonated before she reached
Drøbak, where the captain managed to
beach her. ==Salvage and scrapping==