PR 341 begins between the
International Peace Garden and the town of
Boissevain at a junction with
PTH 10 (John Bracken Highway) in the
Municipality of Boissevain-Morton. It heads due east, having an intersection with Lake William Road (Road 112W,
PR 444 prior to 1992), which leads to
William Lake Provincial Park, before entering the
Municipality of Killarney-Turtle Mountain at the intersection with Road 108W. The highway makes a sharp right turn at an intersection with Road 106W (
PR 346 prior to 1992) before heading south past
Wakopa and the
Mayfair Hutterite Colony. Curving back eastward, PR 341 passes through rural farmland for several kilometres before entering the hamlet of
Lena, coming to an end at an intersection with
PTH 18. The entire length of PR 341 is a two-lane gravel road. ==Major intersections==