MarketMaullinia
Company Profile

Maullinia

Maullinia /ˌmɑˈlɪniə/ is a genus of intracellular, phytomyxid parasites found across the Southern Hemisphere though primarily in Chile, The Prince Edward Islands, South Africa, Australia, and New Zealand. These parasites infiltrate the cells of their brown algal hosts via cytoplasmic extensions called plasmodia that divide synchronously, becoming increasingly multi-nucleate and engulfing the host cell organelles as they grow. Eventually, as the plasmodia fill the entire cell volume, the host cells become hypertrophied and grow to 3- 4x their original size, showing up as swollen appendages or galls on the host tissue at a macroscopic level. These swollen regions will burst alongside the mature Maullinia plasmodia, releasing biflagellated zoospores to the inter- and extracellular space to disperse the infection further. Zoospores can come from sporangial plasmodia, as in M. ectocarpii, or from resting spores, as in M. braseltonii.

Etymology
"Maullinia" refers to the Maullín river near Puerto Montt in Chile, from which the genus was first described. The species epithet for M. ectocarpii was chosen in reference to the host organism genus, Ectocarpus. In M. braseltonii, the species epithet was chosen as a dedication to well-renowned phytomyxid specialist James P. Braselton. == Type species ==
Type species
Maullinia ectocarpii I. Maier, E. R. Parodi, R. Westermeier et D. G. Müller sp. nov. == History of knowledge ==
History of knowledge
For decades, brown algal kelps across the southern hemisphere have been described as having gall-forming infections of unknown origin. The cause of these galls was first investigated purely by chance, when a team of researchers from the University of Konstanz, led by Dr. Ingo Maier, were conducting an epiphytic algal study in Chile. Some specimens of Ectocarpus siliculosus in a Chilean mariculture plantation appeared to have parasitic infections, which piqued the research team's interest. After extensive field and laboratory culture experimentation, a new genus of phytomyxid was described as Maullinia. This was the only phytomyxid parasite of brown algae known to science at the time, and was initially classified as a plasmodiophorid. Close to a decade later, while investigating gall-forming infections on fronds of the kelp Durvillaea antarctica, researchers produced the first evidence of a second Maullinia species. Using 18S rRNA molecular data from the parasites causing these gall-forming infections, they constructed a phylogenetic tree which placed the parasite as a sister species to M. ectocarpii. However, not enough evidence was found to constitute a new species description at this time. A few years later, further molecular and morphological experimentation was carried out, providing enough evidence to classify this new Maullinia species as M. braseltonii. ==Ecology and habitat==
Ecology and habitat
Host interactions The two species of Maullinia has been shown to successfully parasitize a wide range of brown algal hosts, spanning multiple brown algal orders, such as the Ectocarpales, Fucales, and Laminariales Maullinia infections of non-filamentous brown algae primarily target adult hosts, and are mostly absent during their reproductive periods. and as such provide a vector for viral infections to enter the brown alga alongside Maullinia. Nutrition As a parasitic genus, Maullinia derives its nutrition from the host cells it inhabits. Initially, the sole mode of nutrition was thought to be absorptive osmotrophy. Maullinia appears to engulf the host organelles whole as it extends through the cell, with a preference for plastids being clear. This preference could be due to several factors, namely active targeting by Maullinia, the widespread nature of plastids within brown algal cells, or simply a slower digestion process of these complex pigment-containing organelles. Evidence that there is some targeting of plastids comes from the host cell actively producing smaller plastids as the infection proceeds. Outside of the plastid preference, one of the first intracellular structures to be engulfed is the large storage vacuole. This vacuole contains many complex polysaccharides, and as such its consumption provides the infecting Maullinia with a boost in energy. This energy boost near the beginning of the infection, alongside the loss of a key energy source for the host cell, allows Maullinia to expand quickly. Distribution and habitat Maullinia is found across much of the Southern Hemisphere, with infected brown algal populations in Chile, The Prince Edward Islands, South Africa, Australia, and New Zealand currently known. Within these regions Maullinia prevalence increases with latitude, becoming more dominant in southern populations as compared to more tropical ones. This is likely due to a combination of two key factors: the increased density of southern populations and increased Maullinia virulence in cooler waters. While many parasites prefer warmer environments, Maullinia appears to thrive in lower temperatures, having greatest infection rates during the winter months and at greater latitude. The two species of Maullinia appear to have some preference for habitat, based on the brown algal hosts which they each primarily infect. M. ectocarpii prefers sandy, shallow, sheltered regions where more filamentous brown algae tend to thrive. However, M. ectocarpii is a more generalist species and can adapt to more rocky intertidal species such as Durvillaea amatheiae and Durvillaea potatorum. M. braseltonii is much more specialist, and exclusively infects Durvillaea hosts stemming from rocky intertidal habitats. As many Durvillaea species are buoyant, M. braseltonii is often found infecting drift as well. The role of drift in distributing Maullinia and providing a vector for novel infections is critical. Rafting is the primary way by which new populations of brown algae are infected by Maullinia, and this process is supported by the physiological changes infected hosts undergo. With the hypertrophied cells forming galls, a greater buoyancy is achieved in infected individuals, allowing them a wider distribution capability. The likelihood of the mechanism is strengthened by the genetic evidence which links the New Zealand population of Maullinia to the Chile population. The role of rafting, alongside the widespread populations separated by thousands of kilometers, implies that Maullinia may be capable of achieving global distribution along cold-temperate coasts. ==Description==
Description
Life cycle Life cycles in Maullinia are not completely understood, nor has sexual reproduction been observed. M. ectocarpii and M. braseltonii seem to produce two different forms of plasmodia which develop into unique structures and zoospores not yet observed in the other. Outside of these two plasmodial forms, the life cycle is largely identical. Cysts Encystment occurs when a Maullinia biflagellated stage settles on a compatible host, and can begin the infection process. Cysts are ellipsoid to kidney-shaped, being approximately 5.8 ± 0.7 μm long and 2.5 ± 0.2 μm wide. They have chitinous cell walls which break open at one end to allow the adhesorium, an infection apparatus, to extend. The adhesorium has no cell wall and develops from a beak-like shape into a narrow neck of 5 – 7 μm in length which punctures the host cell wall. From here, the cyst contents are injected into the host cell cytoplasm, and plasmodial formation begins. Plasmodia Once cyst contents have been injected into the host cytoplasm, synchronous nuclear divisions occur rapidly to produce large multinucleate plasmodia within the host cell. Each plasmodial nucleus is spherical and contains a distinct, centric nucleolus. Plasmodia continue to grow, via cruciform and non-cruciform mitotic divisions, engulfing host organelles and causing strong host cell hypertrophy. The host nucleus becomes engorged as a result of the hypertrophy, and eventually ceases division. This growth may alter the hosts metabolic functioning, collecting energy-rich compounds within the cell to help feed the Maullinia parasite, although this theory is not proven. After enough plasmodial growth occurs and the host cell is entirely filled, a lobose structure is adopted and plasmodial differentiation occurs. Sporangia & sporangial zoospores (seen in M. ectocarpii) Mature plasmodia transform into a single sporangium, which develops near the host cell wall and becomes attached to it via deposition of a fine, but dense, fibrillar wall-like material. The size and shape of these sporangia are variable both within and across host species, but on average are club-shaped, 40 – 93 μm long and 27–46 μm wide. In Ectocarpus, these sporangia occur in clusters that mirror the hosts' own growth pattern. Each sporangium produces several hundred zoospores. Sporangia produce sporangial zoospores, pyriform cells 4.6 ± 0.7 μm long length, 2.3 ± 0.4 μm wide with thick walls and 2 flagella extending from the broad part of the cell. These flagella are of unequal length, with the longer flagellum being more posteriorly oriented and undulating, while the shorter one is more anteriorly oriented and oblique to the midline. Both flagella have short hair tips along them. Sporangial zoospore nuclei have an abundance of condensed chromatin, but no nucleoli. These zoospores are extremely motile and exit singly from a single apical opening on the sporangium. Once released, they disperse and make contact with hosts to begin encystment again. Resting spores (seen in M. braseltonii) Resting spores allow for survival in antagonistic environments, which allows M. braseltonii to be more specific about which hosts it chooses to infect as a host cell is not needed for resting spore survival. The plasmodium begins resting spore development by concentrating cytoplasm around the nucleus, allowing the thick, 3-layered wall to start forming. Initially, these resting spores are very irregular and globose to elliptic, but become more regularly spherical as they mature. They have no colour, and will wait to release zoospores until conditions are optimal. These zoospores may be differently structured than their sporangial counterparts, but they have yet to be observed. == Practical importance ==
Practical importance
Maullinia has hosts of high commercial importance, such as Macrocystis, and as such have the potential of causing devastating effects if an infection were able to spread into a gametophyte mariculture supporting a Macrocystis kelp farm. ==Species==
Species
Maullinia ectocarpii I. Maier, E. R. Parodi, R. Westermeier et D. G. Müller 2000 • Maullinia braseltonii P. Murúa, F. Goecke et S. Neuhauser 2017 == References ==
tickerdossier.comtickerdossier.substack.com