Obligate anaerobe

The oxygen sensitivity of obligate anaerobes has been attributed to a combination of factors including oxidative stress and enzyme production. Oxygen can also damage obligate anaerobes in ways not involving oxidative stress. Because molecular oxygen contains two unpaired electrons in the highest occupied molecular orbital, it is readily reduced to superoxide () and hydrogen peroxide () within cells. Superoxide, hydrogen peroxide, and hydroxyl radicals are a class of compounds known as reactive oxygen species (ROS), highly reactant products that are damaging to microbes, including obligate anaerobes. The variability in oxygen tolerance of obligate anaerobes (2) is thought to reflect the quantity of superoxide dismutase and catalase being produced. In the absence of oxygen, the mutated samples grew normally. For example, methanogens grow at a redox potential lower than -0.3 V. Sulfide is an essential component of some enzymes, and molecular oxygen oxidizes this to form disulfide, thus inactivating certain enzymes (e.g. nitrogenase). Organisms may not be able to grow with these essential enzymes deactivated. Growth may also be inhibited due to a lack of reducing equivalents for biosynthesis because electrons are exhausted in reducing oxygen. ==Energy metabolism==

Obligate anaerobes convert nutrients into energy through anaerobic respiration or fermentation. In aerobic respiration, the pyruvate generated from glycolysis is converted to acetyl-CoA. This is then broken down via the TCA cycle and electron transport chain. Anaerobic respiration differs from aerobic respiration in that it uses an electron acceptor other than oxygen in the electron transport chain. Examples of alternative electron acceptors include sulfate, nitrate, iron, manganese, mercury, and carbon monoxide. Fermentation differs from anaerobic respiration in that the pyruvate generated from glycolysis is broken down without the involvement of an electron transport chain (i.e. there is no oxidative phosphorylation). Numerous fermentation pathways exist such as lactic acid fermentation, mixed acid fermentation, 2-3 butanediol fermentation where organic compounds are reduced to organic acids and alcohol. The energy yield of anaerobic respiration and fermentation (i.e. the number of ATP molecules generated) is less than in aerobic respiration. This is why facultative anaerobes, which can metabolise energy both aerobically and anaerobically, preferentially metabolise energy aerobically. This is observable when facultative anaerobes are cultured in thioglycolate broth. ==Ecology and examples==

Obligate anaerobes are found in oxygen-free environments such as the intestinal tracts of animals, the deep ocean, still waters, landfills, in deep sediments of soil. Examples of obligatorily anaerobic bacterial genera include Actinomyces, Bacteroides, Clostridium, Fusobacterium, Peptostreptococcus, Porphyromonas, Prevotella, Propionibacterium, and Veillonella. Clostridium species are endospore-forming bacteria, and can survive in atmospheric concentrations of oxygen in this dormant form. The remaining bacteria listed do not form endospores. Obligate anaerobes are also found in the digestive tracts of humans and other animals as well as in the first stomach of ruminants. Examples of obligately anaerobic fungal genera include the rumen fungi Neocallimastix, Piromonas, and Sphaeromonas. == See also ==