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Sonication

Sonication is the act of applying sound energy to agitate particles in a sample, for various purposes such as the extraction of multiple compounds from plants, microalgae, and seaweeds. Ultrasonic frequencies (> 20 kHz) are usually used, leading to the process also being known as ultrasonication or ultra-sonication.

Effects
Sonication has numerous effects, both chemical and physical. The scientific field concerned with understanding the effect of sonic waves on chemical systems is called sonochemistry. The chemical effects of ultrasound do not come from a direct interaction with molecular species. Studies have shown that no direct coupling of the acoustic field with chemical species on a molecular level can account for sonochemistry or sonoluminescence. Instead, in sonochemistry the sound waves migrate through a medium, inducing pressure variations and cavitations that grow and collapse, transforming the sound waves into mechanical energy. ==Applications==
Applications
Sonication can be used for the production of nanoparticles such as nanoemulsions, nanocrystals, liposomes and wax emulsions, as well as for wastewater purification, degassing, extraction of seaweed polysaccharides production of biofuels, crude oil desulfurization, cell disruption, polymer and epoxy processing, adhesive thinning, and many other processes. It is applied in pharmaceutical, cosmetic, water, food, ink, paint, coating, wood treatment, metalworking, nanocomposite, pesticide, fuel, wood product, and many other industries. Sonication can be used to speed dissolution, by breaking intermolecular interactions. It is especially useful when it is not possible to stir the sample, as with NMR tubes. It may also be used to provide the energy for certain chemical reactions to proceed. Sonication is used to remove dissolved gases from liquids (degassing) by sonicating the liquid while it is under a vacuum. This is an alternative to the freeze-pump-thaw and sparging methods. In biological applications, sonication may be sufficient to disrupt or deactivate a biological material. For example, sonication is often used to disrupt cell membranes and release cellular contents. This process is called sonoporation. Small unilamellar vesicles (SUVs) can be made by sonication of a dispersion of large multilamellar vesicles (LMVs). Sonication is also used to fragment molecules of DNA, in which the DNA subjected to brief periods of sonication is sheared into smaller fragments. Sonication is commonly used in nanotechnology for evenly dispersing nanoparticles in liquids. Additionally, it is used to break up aggregates of micron-sized colloidal particles. Sonication can also be used to initiate crystallisation processes and even control polymorphic crystallisations. It is used to intervene in anti-solvent precipitations (crystallisation) to aid mixing and isolate small crystals. Sonication is the mechanism used in ultrasonic cleaning—loosening particles adhering to surfaces. In addition to laboratory science applications, sonicating baths have applications including cleaning objects such as spectacles and jewelry. Sonication is used in food industry as well. Main applications are for dispersion to save expensive emulgators (mayonnaise) or to speed up filtration processes (vegetable oil etc.). Experiments with sonication for artificial ageing of liquors and other alcoholic beverages were conducted . Soil samples are often subjected to ultrasound in order to break up soil aggregates; this allows the study of the different constituents of soil aggregates (especially soil organic matter) without subjecting them to harsh chemical treatment. Sonication is also used to extract microfossils from rock. An ultrasonic bath or an ultrasonic probe system is used for extraction. For instance, this technique was suggested to remove isoflavones from soybeans and phenolic compounds from wheat bran and coconut shell powder. The outcomes differ for every raw material and solvent utilized and the other extraction techniques. Acoustic or ultrasonic cavitation is the basis for the operation of ultrasound-assisted extraction. ==Equipment==
Equipment
Substantial intensity of ultrasound and high ultrasonic vibration amplitudes are required for many processing applications, such as nano-crystallization, nano-emulsification, Finding the optimum operation condition for this equipment is a challenge for process engineers and needs deep knowledge about side effects of ultrasonic processors. ==See also==
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