10-CHO-THF is produced from
methylenetetrahydrofolate (CH2H4F) via a two step process. The first step generates
5,10-methenyltetrahydrofolate: :CH2H4F + NAD+ \rightleftharpoons CH2H2F + NADH + H+ In the second step 5,10-methenyltetrahydrofolate undergoes hydrolysis: :CH2H2F + H2O \rightleftharpoons CHO-H4F + The latter is equivalently written: :
5,10-methenyltetrahydrofolate + H2O \rightleftharpoons 10-formyltetrahydrofolate 10-CHO-THF is also produced by the reaction :ATP +
formate + tetrahydrofolate \rightleftharpoons ADP + phosphate + 10-formyltetrahydrofolate This reaction is catalyzed by
formate-tetrahydrofolate ligase. It can be converted back into
tetrahydrofolate (THF) by
formyltetrahydrofolate dehydrogenase or THF and formate by
formyltetrahydrofolate deformylase. ==References==