Whey is left over when milk is
coagulated during the process of cheese production. Coagulation can happen by adding
acid or
rennet. It is a 5% solution of
lactose in water and contains the water-soluble proteins of milk as well as some
lipid content. Processing can be done by simple drying, or the relative protein content can be increased by removing the lactose, lipids and other non-protein materials. The main method to extract protein from whey is membrane filtration. A variety of membrane pore sizes can be used to selectively let different components of whey go through or be retained. Whey can be passed through "microfiltration" which blocks bacteria, casein
micelles, and fat, then passed through "ultrafiltration" (UF) which blocks proteins. The part that does not go through UF is
spray-dried into a concentrated whey protein. There are also other ways to concentrate protein using filtration membranes. Ion exchange chromatography is another major method for whey protein extraction. Methods in development include aqueous two-phase extraction and magnetic separation.
Microbial production , made with non-animal whey protein produced by microbes Microbes have been engineered to produce proteins similar or even "bioidentical" to whey. Various companies offer microbe-produced whey and cheese; however, none of these companies stipulate the protein composition of their products, but they do contain some of the
genes needed to make whey proteins. ==Composition==