In 1534, William Stafford secretly wed, as her second husband,
Mary Boleyn (c. 1499 – 1543), sister of
King Henry VIII's second wife,
Anne Boleyn. Mary Boleyn is said to have been pregnant at the time of her marriage to Sir William Stafford and they may have had two children: • Edward Stafford (1535–1545). • Anne Stafford (b. 1536?), possibly named in honour of Mary's sister, Queen
Anne Boleyn. However if these children did exist, nothing further is known of them. Stafford married secondly, in 1545,
Dorothy Stafford (d. 22 September 1604), daughter of
Henry Stafford, 1st Baron Stafford, and
Ursula Pole, by whom he had three sons and three daughters: •
Elizabeth Stafford (1546 – 6 February 1599), who married firstly,
Sir William Drury (1550–1590), by whom she had issue. She married secondly
Sir John Scott. •
Sir Edward Stafford (1552–1604) of
Grafton, who married firstly, Roberta Chapman (d. 1578), the daughter of Alexander Chapman of
Rainthorpe Hall, Norfolk, by whom he had a son and two daughters, and secondly, on 29 November 1597,
Douglas Sheffield (1547–1608), daughter of
William Howard, 1st Baron Howard of Effingham, and sister of
Charles Howard, 1st Earl of Nottingham. • Ursula Stafford (b. 1553), who married
Richard Drake (d. 11 July 1603) of
Esher, Surrey,
equerry to
Elizabeth I, third son of John Drake (d. 1558), esquire, of
Ash in the parish of
Musbury, Devonshire, and brother of
Bernard Drake, by whom she had a son, Francis Drake (d. 1633). •
William Stafford (1554–1612), conspirator, who about 1593 married Anne Gryme (d. 1612), daughter of Thomas Gryme of
Antingham, Norfolk, by whom he had a daughter, Dorothy Stafford, and a son,
William Stafford (1593–1684). • Sir John Stafford of Marlwood Park (January 1556 – 28 September 1624),
Thornbury, Gloucestershire, who married firstly, Bridget Clopton (d. March 1574), the daughter of William Clopton of
Kentwell Hall, by whom he had a son, and secondly, on 29 January 1580, Millicent Gresham (buried 24 December 1602), the daughter of Edmund Gresham (buried 31 August 1586) and Joan Hynde, by whom he had no issue. • Dorothy Stafford, who likely died in infancy. ==Ancestry==