Main • Lucky (voiced by
Pamela Adlon and
Debi Mae West) is the most plucky of the puppies and is unique in having his only spots in the shape of a horseshoe on his back. He is the leader of the main pups and is very adventurous and determined to do whatever he thinks is right. But he is very obsessed with TV and he is usually the one at fault when the main pups get into trouble. • Cadpig (voiced by
Kath Soucie) is the smallest of the puppies and one of the most intelligent, if not deluded somewhat by her abstract state of mind. She is unique in having long floppy ears and a big head. She has a kind-hearted personality, is quite strong for her size and can have a very strong temper. • Rolly (voiced by Kath Soucie) is always hungry; almost all his decisions are based on food, which sometimes gets him and the pups into trouble. However, he is a cheerful and diligent sibling, especially when it comes to his incredible sense of smell. He's also very timid and is an easy target for bullies due to him being such a foodie. • Spot (voiced by
Tara Strong) (credited as Tara Charendoff) is a chicken who wants to become a dalmatian. She is the most intelligent of the main four and is a voice of reason for the foursome, but is more often than not ignored. She is easily scared and is prone to literally bouncing off the walls. But she is a great dancer and appears to be good at math. She is also able to fly to some extent. In certain episodes, she acts as a private investigator and sports an alter-ego by the name of Pullet Marlowe. • Roger (voiced by
Jeff Bennett) and Anita Dearly (voiced by Kath Soucie) are owners of the 101 Dalmatians and the Dearly Farm. •
Cruella de Vil (voiced by
April Winchell and
Tress MacNeille in 2 episodes) is now a corporate criminal and most of her plans revolve around getting richer, tormenting the dalmatians and plotting to steal the Dearly Farm. Her frequent catchphrase in the series is "Memo to myself", followed by whatever her current scheme is or sometimes her emotional state, uttered into her personal recording device. • Horace (voiced by
David Lander) and Jasper (voiced by
Michael McKean) are Cruella's henchmen. They are usually hired to carry out her plans and generally fail, due to their lack of intelligence.
Supporting • Pongo (voiced by
Kevin Schon) is the father of fifteen puppies, the adopted father of the eighty-four orphaned pups and the husband of Perdita. He is Roger's devoted pet. • Perdita (voiced by
Pam Dawber) is the mother of fifteen puppies, the adoptive mother of eighty-four orphaned pups and the wife of Pongo. She is Anita's beloved pet. • Nanny (voiced by
Charlotte Rae) is Dearlys' caretaker who helps Roger and Anita around the Dearly Farm, also helps with feeding the dalmatians from the huge Chow Tower that she operates with a bike. • Mooch (voiced by
Danny Cooksey) is an adolescent
sheepdog mix who is the bully of the farm. He can sometimes be intimidating, but deep down he is very sensitive and nice. • Two-Tone (voiced by Tara Strong) is one of the dalmatian puppies and Mooch's ex-girlfriend and second-in-command. She is depicted as half-black with white spots and half white with black spots. She is a tomboy who loves fashion and has a crush on Lucky. • Dipstick (voiced by
Thom Adcox-Hernandez) is a male dalmatian puppy who is the most dimwitted of Mooch's gang. He is constantly infested with fleas. In some episodes, he is has a soft side. • Wizzer (voiced by Pamela Adlon in "Purred It Through the Grapevine" and
Christine Cavanaugh for the rest of the series) is a male dalmatian puppy and bladder control issues who is part of Mooch's gang. • Tripod (voiced by
Toran Caudell) is a male dalmatian puppy who serves as Lucky's friendly rival. He has spotted ears, a green headband and is missing his front left leg. • Scorch (voiced by
Frank Welker) is Cruella's pet
ferret who has an appetite for Spot. • The Colonel (voiced by
Jim Cummings) is an
old English sheepdog and commanding officer of the Bark Brigade. • Sergeant Tibbs (voiced by Jeff Bennett) is a cat and trusted ally of the Colonel. • Captain (voiced by Frank Welker) is a horse on the Dearly Farm who is usually seen helping Nanny. • Lieutenant
Pug (voiced by Jeff Bennett) is one of the training officers of the Bark Brigade. He is
paranoid about a potential feline invasion. He gets very brutal with the main pups and has a habit of insulting them, and has
narcolepsy. • Thunderbolt (voiced by Frank Welker) is a
German shepherd and Lucky's favorite superhero. • Cornelia (voiced by Tress MacNeille) is Spot's overprotective mother who wants her to behave like a chicken. • Lucy (voiced by
Paddi Edwards) is a goose who gets grumpy when the dalmatians play in her pond at Hiccup Hole. • Swamp Rat (voiced by Jeff Bennett) is a
rat salesman who lives in the swamp. • Steven the
Alligator (voiced by Frank Welker) is Swamp Rat's associate and is fixated on eating Spot. • Cydne (voiced by Frank Welker) is a
snake who lives in the swamp and Swamp Rat's other associate. • Mayor Ed Pig (voiced by Jim Cummings) is a
pig who is the mayor of the animals on the Dearly Farm. • Dumpling (voiced by
Christine Cavanaugh) is Mayor Ed's daughter who has an unrequited crush on Lucky. • Princess (voiced by April Winchell in "The High Price of Fame" and "Shake, Rattle and Woof" and
Cree Summer for the rest of the series) is a
cow who lives on the Dearly Farm. • Duchess (voiced by
Marla Gibbs) is a cow and a companion of Princess. ==Production==