The current season saw an expansion from 16 teams. At the end of the
previous season,
Lokomotiv,
Metallurg and
Olympic were relegated to the
Uzbekistan Pro League. The newly-promoted teams from the
2024 Uzbekistan Pro League were
Mash'al Mubarek,
Shurtan Guzar,
Kokand 1912,
Khorazm and
Bukhara. The draw for the 2025 Uzbekistan Super League was made on 1 February. In March,
Uzbekistan Professional Football League established a partnership with
Macron, bringing the number of league sponsors to six. The date of the 12th round of the Super League schedule has been changed due to the international matches of the Uzbekistan national team to be held from 2 to 10 June. On 7 August,
Bunyodkor Stadium hosted a concert by singer
Jennifer Lopez titled "
Up All Night: Live in 2025". In connection with this, the match between
Bunyodkor and
Shurtan in the 17th round was moved to
Jar stadium. After the program, the stadium's grass was seriously damaged. Given the 3-4 months it would take to restore the grass, the home matches of
Bunyodkor were temporarily moved to
Jar stadium. On 18 August, the newly built
Olympic City Stadium was opened in Tashkent, and it was decided that FC Bunyodkor would play at this stadium until next season. In the end-of-season play-offs, Kokand 1912, Bukhara, Khorezm and Mashal retained their places in the Super League. Shurtan was relegated to the Pro League. Lokomotiv returned to the Super League.
Managerial changes ==Foreign players==