Participants in the competition were determined by regional competitions and play-offs matches staged in 2023. The top three teams of that year's
Six Nations and
Pacific Four series were placed in WXV 1. The fourth-placed team in both tournaments were placed in WXV 2 alongside that year's
African,
Asian and
Oceanian champions; their runners-up were placed in WXV 3 alongside the sixth-placed Six Nations team. The South American play-off was a two-match series on 5 and 9 July in
Medellín, Colombia. It was preceded by the
2023 Americas Rugby Trophy, the first South American tournament for women's national teams sanctioned by
World Rugby. A match in the tournament between Brazil and Colombia was decided by a late try by Juliana Soto, helping Colombia win 18–15. In the opening match, Colombia scored thrice before half-time, including a double from Valentina Álvarez. Brazil mounted a comeback in the second half though, as the pressure of two
yellow cards on Colombia's defence helped facilitate a controversial late try by Lohana Valente; evidence that she had grounded the ball was unclear. However, the attempt to
convert it failed, and the match ended with Colombia leading the series by a single point. Two late
penalty tries for Brazil were not enough to overcome the 54–42 aggregate score, and thus Colombia earned qualification to WXV 3. The European play-off was a single match between
Italy and
Spain on 22 July in
Piacenza, Italy. Italy played their first game at the and made a comfortable four-try defeat of Spain, and consequently earning qualification to WXV 2. Despite a notable offensive effort early in the second half, Spain failed to score a single point in the match, settling for a place in WXV 3.
Play-offs WXV 3 final qualification WXV 2/WXV 3 play-off match Notes ==Match officials==