There have been numerous biological studies on the Etruscan origins, the oldest of which dates back to the 1950s when research was still based on blood tests of modern samples, and DNA analysis (including the analysis of ancient samples) was not yet possible. It is only in very recent years, starting in 2019, with the development of
archaeogenetics, that comprehensive studies containing the
whole genome sequencing (WGS) of Etruscan samples have been published, including
autosomal DNA and
Y-DNA, autosomal DNA being the "most valuable to understand what really happened in an individual's history", as stated by geneticist
David Reich, whereas previously studies were based only on
mitochondrial DNA analysis, which contains less and limited information. The direct testing of ancient Etruscan DNA supports a deep, local origin, while the testing of modern samples as a proxy for Etruscans has proven to be rather inconclusive and inconsistent. A 2019 genetic study published in the journal
Science analyzed the
autosomal DNA of 11
Iron Age samples from the areas around Rome, including for the first time the whole genome sequencing (WGS) of some samples from Etruscan tombs, and concluded that Etruscans (900-600 BC) and the
Latins (900-200 BC) from
Latium vetus were genetically similar, and Etruscans also had
Steppe-related ancestry despite speaking a
pre-Indo-European language. In the collective volume
Etruscology published in 2017, British archeologist Phil Perkins provides an analysis of the state of DNA studies and writes that "none of the DNA studies to date conclusively prove that Etruscans were an intrusive population in Italy that originated in the Eastern Mediterranean or Anatolia" and "there are indications that the evidence of DNA can support the theory that Etruscan people are autochthonous in central Italy".
Archeogenetics and ancient DNA A 2021 study (Posth et al. 2021) focused entirely on the question of the origins of the Etruscans, analyzed the
Y-chromosome,
mitochondrial DNA, and
autosomal DNA of 82 ancient samples from Etruria (Tuscany and Latium) and southern Italy (Basilicata) spanning from 800 BC to 1000 AD, including 48 Iron Age individuals. The study observed that in the samples of Etruscan individuals from Tuscany and Lazio the ancestral component
Steppe was present in the same percentages found in the previously analyzed samples of Iron Age Latins, and added that in the DNA of the Etruscans was completely absent a signal of recent admixture with Anatolia or the Eastern Mediterranean. The study concluded that the Etruscans were autochthonous and they had a genetic profile similar to that of their early Iron Age Latin neighbors. Both Etruscans and Latins belonged firmly to the European cluster: 75% of the samples of Etruscan male individuals were found to belong to haplogroup
R1b, especially R1b-P312 and its derivative R1b-L2 whose direct ancestor is R1b-U152. Regarding mitochondrial DNA haplogroups, the most prevalent was largely H, followed by J and T. Uniparental marker data and autosomal DNA data from samples of Iron Age Etruscan individuals suggest that Etruria received migrations with a large ancestral Steppe component during the
2nd millennium BC, related to the spread of
Indo-European languages, starting with the
Bell Beaker culture, and that these migrations merged with populations of the oldest pre-Indo-European layer present since at least the Neolithic period, but it was the latter's language that survived, a situation similar to what happened in the
Basque region of northern
Spain. The study also concluded that the samples analyzed show that the Etruscans kept their genetic profile unchanged for almost 1000 years, indicating the sparse presence in Etruria of foreigners, and that a demographic change in Etruria occurred only from the
Roman imperial period, in which there is the intermixture into the local population of ancestral components from the Eastern Mediterranean Sea. Analysis of samples of individuals who lived in the Roman imperial period and those of the Medieval Age also suggest that the genetic landscape of present-day central Italy was formed largely around 1000 years ago after the
Barbarian invasions, and that the arrival of the Germanic
Lombards in Italy contributed to the formation of the gene pool of the modern population of Tuscany and northern Latium. These results are largely in line with previous mtDNA results from 2004 (in a smaller study also based on ancient DNA), and contradictory to results from 2007 (based on modern DNA). The 2004 study was based on
mitochondrial DNA (mtDNA) from 80 bone samples, reduced to 28 bone samples in the analysis phase, taken from
tombs dating from the seventh century to the third century BC from
Veneto,
Tuscany,
Lazio and
Campania. This study found that the ancient DNA extracted from the Etruscan remains had some affinities with modern European populations including
Germans,
English people from Cornwall, and Tuscans in Italy. The study was marred by concerns that mtDNA sequences from the archeological samples represented severely damaged or contaminated DNA; however, subsequent investigation showed that the samples passed the most stringent tests of DNA degradation available.
Older studies based on modern samples only An mtDNA study from 2007, by contrast, earlier suggested a
Near Eastern origin. Achilli
et al. (2007) found in a modern sample of 86 individuals from Murlo, a small town in southern Tuscany, an unusually high frequency (17.5%) of supposed Near Eastern mtDNA haplogroups, while other Tuscan populations do not show the same striking feature. Based on this result Achilli concluded that "their data support the scenario of a post-Neolithic genetic input from the Near East to the present-day population of Tuscany, a scenario in agreement with the Lydian origin of Etruscans". This research has been much criticized by archeologists, etruscologists and classicists. In the absence of any dating evidence, there is no direct link between this genetic input found in Murlo and the Etruscans. Furthermore, there is no evidence that these mtDNA haplogroups found in Murlo might be proof of an eastern origin of the Etruscans, as some of these mtDNA haplogroups have been found in other studies as early as the
Neolithic and
Aeneolithic in Italy and Germany. A recent Y-DNA study from 2018 on a modern sample of 113 individuals from
Volterra, a town of Etruscan origin, Grugni at al. keeps all the possibilities open, although the autochthonous scenario is the most supported by numbers, and concludes that "the presence of
J2a-M67* (2.7%) suggests contacts by sea with Anatolian people, the finding of the Central European lineage
G2a-L497 (7.1%) at considerable frequency would rather support a Northern European origin of Etruscans, while the high incidence of European R1b lineages (R1b 49.8%, R1b-U152 24.5%) cannot rule out the scenario of an autochthonous process of formation of the Etruscan civilization from the preceding Villanovan society, as suggested by Dionysius of Halicarnassus". In Italy Y-DNA J2a-M67*, not yet found in Etruscan samples, is more widespread on the Adriatic Sea coast between
Marche and
Abruzzo, and not in those where once lived the Etruscans, and in the study has its peak in the Ionian side of
Calabria. In 2014, a late Bronze Age
Kyjatice culture sample in
Hungary was found to be J2a1-M67, a couple of J2a1b were found in Late Neolithic samples from the
LBK culture in
Austria, a J2a1a was found in a Middle Neolithic
Sopot culture sample from
Croatia, In 2019, in a Stanford study published in Science, two ancient samples from the Neolithic settlement of Ripabianca di Monterado in the
province of Ancona, in the Marche region of Italy, were found to be Y-DNA J-L26 and J-M304. Therefore, Y-DNA J2a-M67 may be likely in Italy since the Neolithic and can't be the proof of recent contacts with Anatolia. In any case, J2a-M67 was not found among the Etruscan samples, unlike G2a-L497 and R1b-U152 who were actually found in the Etruscan individuals in really significant percentages. A 2019 study, based on autosomal DNA of 1616 individuals from all 20 Italian administrative regions, concludes that Tuscans join the northern Italian cluster, close to the inhabitants of
Liguria and
Emilia-Romagna. A 2013 study, based on uniparental markers of 884 unrelated individuals from 23 Italian locations, had shown that the structure observed for the paternal lineages in continental Italy and Sicily suggests a shared genetic background between people from Tuscany and Northern Italy from one side, and people from Southern Italy and the Adriatic coast from the other side. The most frequent Y-DNA haplogroups in the group represented by populations from North-Western Italy, including Tuscany and most of the Padana plain, are four R1b-lineages (R-U152*, R-M269*, R-P312* and R-L2*). ==References==