Early years In the first half of 2006,
FC Twente became the first professional Dutch football club to create a women's section, appointing to set up its women branch. Starting in 2007, the idea was to offer girls and women professional training six days a week and eventually build girls and women's teams. At that time, women's football in the Netherlands was amateur, and most talented female players left for Germany and other countries with professional leagues. In November 2006, the club held a presentation of its women's football department proposal to other amateur clubs and the
Netherlands women's national football team coach
Vera Pauw. On 21 January 2007, after holding a players selection trials in two locations (
Hengelo and
Enschede) attended by 575 players, the club women's section was officially established, consisting of three teams, the senior first team and two youth teams. In March 2007, the
Royal Dutch Football Association (KNVB) announced FC Twente as one of the six teams to participate in the inaugural
2007–08 season of the
Eredivisie Vrouwen, the professional women's Dutch league.
Eredivisie (2007–2012) For the first Eredivisie season, coach Kok-Willemsen built a 22 player squad formed with eight players coming from , two players from the
Belgian First Division, and three players from the
German Bundesliga. The club played its first official match on 29 August 2007, also the first-ever Eredivisie game at home at the
Arke Stadion in front of 5.500 spectators, losing 2–3 to
SC Heerenveen. Despite having a poor 2007–08 league season, finishing in fifth place (out of six teams), the club had a good run in the
KNVB Women's Cup (Dutch Cup), winning its first trophy by beating
FC Utrecht 3–1 in the final. The 2008–09 season was difficult, as the team had many injured players during the season, and the club finished the league again in fifth place out of seven teams. In the Dutch Cup, the KNVB decided to take all Eredivisie clubs out of the competition in the Round of 16 to have the professional players prepared for the
UEFA Euro 2009 tournament. The Tukkers narrowly missed a double, losing the Dutch Cup final
on penalties to
ADO Den Haag. In the
2014–15 UEFA Champions League, the club drew French team
Paris Saint-Germain as opponents in the Round of 32 and lost both legs (1–2 and 0–1). The club won its second Dutch Cup, defeating Ajax 3–2 in the final.
Eredivisie (2015–present) In 2015, the BeNe League dissolved, and the Eredivisie was re-introduced. The club won the Dutch championship for the fourth consecutive year after a 3–0 win over
PEC Zwolle on 20 May 2016. In the
2015–16 UEFA Champions League, three wins in the qualification round got the team to the Round of 32, being drawn to play against German club
Bayern Munich. After a 1–1 first leg draw at home in the Grolsch Veste, the team drew the second leg 2–2 in Germany, advancing on
away goals rule to the Round of 16 for the first time. The next opponents Spanish club
Barcelona won both legs (0–1 and 0–1) in the Round of 16. In the Dutch Cup, the team was eliminated by
Ajax on penalty shoot-out, following a 0–0 draw in the quarterfinals. ,
Lynn Wilms and
Joëlle Smits (L-R) celebrating with the Eredivisie championship shield in 2019 Ahead of the 2016–17 season,
Tommy Stroot was appointed as coach. The club ended the
2016–17 Eredivisie as runners-up behind Ajax. At the
2016–17 UEFA Champions League, three wins in the qualification round got the team to the Round of 32, being drawn to play against Czech club
Sparta Praha. In the first leg at home, the club won its first Champions League main tournament match by 2–0, and, after a 3–1 win on the second leg, advanced to the Round of 16 for the second time. The club next faced Spanish club Barcelona for the second consecutive year in the Round of 16. After losing both legs (0–1 and 0–4) it was eliminated from the competition. In the Dutch Cup, the team lost 2–3 to PEC Zwolle in the quarterfinals. In the 2017–18 season, Twente was in first place at the end of the regular season portion of the competition, but finished second in the championship playoff to Ajax.
Joëlle Smits led the team with 20 goals. FC Twente returned to the top of the league in the
2018–19 season, earning is sixth national championship. Smits again led the team in scoring with 25 goals in Eredivisie play, and
Renate Jansen had 18 goals. The two forwards combined for 61 percent of the team's 70 goals. By winning the league title, the club qualified for the
2019–20 UEFA Champions League. In the Dutch Cup, Twente lost in the quarterfinals to
Feyenoord, 0–3. In the
2019–20 UEFA Champions League, Twente hosted its qualifying round group at the Sportpark Schreurserve in August 2019, prior to Eredivisie league play. Twente won its group, defeating Armenian club Alashkert 8–0, drawing with Turkish club
Beşiktaş, then defeating Polish club
Górnik Łęczna 2–0 to qualify for the 32-team knockout bracket. Twente drew Austrian club
St. Pölten. Each team won on the road, with Twente winning in
St. Pölten 4–2, with new striker
Fenna Kalma scoring a hat trick. St. Pölten won in Enschede 2–1, and Twente advanced on aggregate goals, 5–4. In the round of 16, Twente lost to
VfL Wolfsburg by an aggregate goal score of 7–0. The
2019–20 Eredivisie season was suspended in March 2020 due to the
COVID-19 pandemic, and no league champion was crowned. When matches were halted, Twente was in third place, two points behind Ajax. That finish meant Twente did not qualify for the following year's Champions League. In the Dutch Cup, Twente won its only match, defeating lower-level club Nooit Gedacht of
Geffen 12–0 in the round of 16. A quarterfinal match against
SC Heerenveen planned for March 2020 was suspended due to the COVID-19 pandemic. In
2020–2021, Twente returned to the top, narrowly. After the first half of the season, Twente was in first place by one point over PSV, which along with Ajax were clear top three teams in the league, based on results. After the championship playoff, Twente again held a one-point lead over PSV, and the two teams qualified for the
2021–22 UEFA Champions League. Twente clinched the title in the penultimate week, defeating the weaker ADO Den Haag as Ajax bested PSV. Twente had three of the four top goal scorers in the league, in
Renate Jansen,
Fenna Kalma, and
Anna–Lena Stolze, respectively. All three players averaged more than one goal per two games. All three trailed the team's former top scorer, Joëlle Smits, who had transferred to PSV in 2019. In mid-2021, Stroot left to coach
VfL Wolfsburg. Twente again won the Eredivisie in the
2021–22 season under new coach
Robert de Pauw. The league switched to a triple round robin schedule, ending the championship playoff round format. Twente finished six points clear of Ajax and led the league by scoring 95 goals in 24 matches. The 26 goals conceded was second only to Ajax. Kalma led the league with 33 goals, which tied or bested the goal count of the bottom five teams in the standings. She had
four hat tricks, a total matched by all the other players in the league. Jansen had 17 goals and
Kayleigh van Dooren had 13 goals, ranking third and fourth in the league, respectively. In Champions League play, Twente hosted and won its
first round of the Champions Path qualifying, defeating Tbilisi Nike and
Spartak Subotica in August, before the start of the Eredivisie regular season. However, Twente lost to
Benfica in the
second round, with the decisive match being a 4–0 loss in the second leg in
Seixal, Portugal. In that game, Twente conceded a hat trick to Benfica's
Cloé Lacasse. Twente also lost early in the KNVB Women's Cup. de Pauw lasted just one season with the club, moving on to coach German club
Bayer 04 Leverkusen.
Joran Pot was announced as the team's coach on 26 January 2022, coming from PEC Zwolle. Pot won several championships, including a treble in his final season in 2024–25, before leaving to coach the Dutch under-16 national boys team. became the team's new coach in 2025. ==Competitive record==