Margolin's earliest acting roles were in a commercial for
Zest and several installments in the
soap opera The Edge of Night. In 1961 at the age of 18, while a prop assistant at the
New York Shakespeare Festival, Margolin won a pivotal Broadway stage role as Anna in
Morris West's
Daughter of Silence, beating 200 other applicants. Despite mixed reaction to the play, critics unanimously praised Margolin's performance and she went on to be nominated for a Tony Award. Her performance as Anna charmed director Frank Perry, Margolin was immediately cast to played her first movie role as the female lead in
David and Lisaand traveled to Argentina in 1964 to feature in
The Eavesdropper, where upon her return she signed a 1-film-a-year contract with
20th Century Fox. She co-starred with
Marlon Brando in 1965's
Morituri and with
Steve McQueen in the western
Nevada Smith. By 1967, she was considered by the
Baltimore Sun one of Hollywood's "brightest new stars," by then having featured in around five films. Numerous film studios made efforts to commit her to a long-term contract. Later that year, she also played Wanda in the movie
Enter Laughing, as the love interest of the character David Kolowitz, played by
Reni Santoni in his first leading role. In
Take the Money and Run (1969), she played the love interest of the bumbling thief played by Woody Allen, and in
Annie Hall (1977), she played the social-climbing wife of Allen's character. In 1979, Margolin co-starred with
Roy Scheider in director
Jonathan Demme's thriller
Last Embrace. Margolin's last film appearance was in
Ghostbusters II in 1989, and her last television roles were in an episode of
Murder, She Wrote ("Deadly Misunderstanding") and in
Columbo: Murder in Malibu in 1990. == Personal life ==