Main characters •
Mickey Mouse (voiced by
Bret Iwan): The optimistic and easygoing leader of The Sensational Six. Donald's best friend and Minnie's boyfriend, he is very patient and caring, especially towards his pet dog Pluto. •
Minnie Mouse (voiced by
Kaitlyn Robrock): Donald's stuffed lion. •
Daisy Duck (voiced by
Tress MacNeille): Daisy Bo Peep's sheep. • Francis (vocal effects provided by
Dee Bradley Baker): Daisy's pet frog. •
Goofy (voiced by
Bill Farmer): A small, flying, Mickey-shaped robot extension of the Mousekedoer who delivers the Mouseketools for every episode. Despite being voiced by
Rob Paulsen in the original series, Toodles doesn't speak in the first season. • Little Helper (vocal effects provided by
Dee Bradley Baker): Pete is a large cat who is a friend and occasional foe of Mickey's depending on the alter ego. Some of his alter egos have appeared as Mystery Mouskepals. • Buster (vocal effects provided by
Dee Bradley Baker): Professor Von Drake's pet robot dog. •
Clarabelle Cow (voiced by
April Winchell): A hen who mostly speaks chicken language. • Hilda (voiced by
April Winchell): A female hippopotamus who plays several sports and has different alter egos as a Mystery Mousekepal. • Leapin' Leonardo (vocal effects provided by Brock Powell): A playful purple
dragon who previously debuted in
Mickey Mouse Funhouse. • Coco the Monkey (vocal effects provided by Dee Bradley Baker): Goofy's monkey friend from the jungle who was originally seen in the original series. • Baby Elephant (vocal effects provided by
Dee Bradley Baker) A baby elephant that first appears as a Mouseketool. It might be implied to be Bubbles from the original Clubhouse series. The Baby Elephant later appeared as a Mystery Mousekepal in "Daisylocks and the Three Bears" when it was summoned to help wake Papa Bear. •
Duffy the Disney Bear: A wind-up cuckoo bird who lives in Minnie's Clubhouse. She previously debuted in ''
Minnie's Bow-Toons''. • The Three Bears: A family of three bears. • Papa Bear (vocal effects provided by
Bill Farmer): • Mama Bear (vocal effects provided by
Dee Bradley Baker): A trio of gophers who originally lived in the holes that were accidentally used for the golf holes. One Gopher was the Mystery Mousekepal in "SPOTS the Beagle-Bot" when Pluto had to teach SPOTS how to point at it. • Martian Mickey (voiced by Bret Iwan): The
Martian counterpart of Mickey Mouse who lives on
Mars. • Martian Minnie (voiced by
Kaitlyn Robrock): A horse. In "Minnie's Rubber Ducky Doozy", Horace appeared as a Mystery Mousekepal named Horace Seahorse who is fluent in
seahorse language and is shown to be half-seahorse. • Millie and Melody Mouse (voiced by Vivian Vencer and Stella Edwards respectively): Minnie's nieces. They first appear in "Mickey's Movie Night" as the Mystery Mousekepals where they keep everyone at Picture Pete's Drive-In Movie Theater entertained while Picture Pete works on winding up the
Kansas City Mickey film reel. • Figaro (vocal effects provided by
Frank Welker): Minnie's pet cat.
Guest characters • Cat Family: A swarm of bees whom Donald once angered. • Mousekedoer Voice Announcer (voiced by
Dee Bradley Baker) A hi-tech van that serves as transportation for the Go-Getters. It has an A.I. system. • Seahorses (vocal effects provided by
Dee Bradley Baker): A talking
earthworm who previously debuted in the interstitial
Mickey Mouse Mixed-Up Adventures spin-off ''Chip 'n Dale's Nutty Tales''. • Clam (voiced by
Dee Bradley Baker): A bunch of frogs who live near Donald's Clubhouse. • Baby Red Bird (vocal effects provided by
Tress MacNeille): A little red baby bird. ==Clubhouses==