Speckle patterns have been used in a variety of applications in microscopy, imaging, and optical manipulation. When lasers were first invented, the speckle effect was considered to be a severe drawback in using lasers to illuminate objects, particularly in
holographic imaging because of the grainy image produced. Researchers later realized that speckle patterns could carry information about the object's surface deformations, and exploited this effect in
holographic interferometry and
electronic speckle pattern interferometry.
Speckle imaging and
eye testing using speckle also use the speckle effect. Speckle is the chief limitation of coherent
lidar and coherent imaging in
optical heterodyne detection. In the case of near field speckles, the statistical properties depend on the light scattering distribution of a given sample. This allows the use of near field speckle analysis to detect the scattering distribution; this is the so-called
near-field scattering technique. When the speckle pattern changes in time, due to changes in the illuminated surface, the phenomenon is known as
dynamic speckle, and it can be used to measure activity, by means of, for example, an optical flow sensor (optical computer mouse). In biological materials, the phenomenon is known as biospeckle. In a static environment, changes in speckle can also be used as a sensitive probe of the light source. This can be used in a wavemeter configuration, with a resolution around 1
attometre, (equivalent to 1 part in 1012 of the wavelength, equivalent to measuring the length of a
football field at the resolution of a single
atom) and can also stabilise the wavelength of lasers or measure polarization. The disordered pattern produced by speckle has been used in
quantum simulations with
cold atoms. The randomly-distributed regions of bright and dark light act as an analog of disorder in
solid-state systems, and are used to investigate
localization phenomena. In fluorescence microscopy, a sub-diffraction-limited resolution can be obtained in 2D from saturable/photoconvertible pattern illumination techniques like stimulated emission depletion (
STED) microscopy, ground state depletion (
GSD) microscopy, and reversible saturable optical fluorescence transitions (RESOLFT). Adapting speckle patterns for use in these applications enables parallel 3D
super-resolution imaging. ==Mitigation==