On Earth-10955, the
Amazing Spider-Man accidentally shatters an artifact called the Tablet of Order and Chaos while trying to prevent
Mysterio from stealing it. While Mysterio escapes with one Tablet fragment, Spider-Man is approached by
Madame Web, who reveals that the others have been scattered across this and three other realities across the
multiverse. To restore balance, Madame Web recruits him,
Spider-Man Noir,
Spider-Man 2099, and the
Venom symbiote-empowered
Ultimate Spider-Man to retrieve the fragments from their native dimensions. After the Spider-Men each collect their first fragment with no difficulty, Madame Web warns them that the fragments can grant incredible powers and must not be allowed to fall into the wrong hands. is shown fighting
Juggernaut. While searching for their second fragment, the Spider-Men discover that they have already been found by supervillains. The Amazing Spider-Man is captured by
Kraven the Hunter and taken to a jungle, where he is forced to overcome a series of trials for a fragment. However, Kraven reneges on their deal and uses it to gain superpowers. Spider-Man Noir pursues
Hammerhead, who is driven mad with power after using a fragment he had found to fuse his arms with his guns and plots to overthrow his boss,
Norman "The Goblin" Osborn. Spider-Man 2099 is attacked by the
Hobgoblin, a
cyborg mercenary who uses his fragment to increase his psy-powers and create illusions to torment the hero. The Ultimate Spider-Man battles
Electro to prevent him from destroying a hydro-electric
dam, where the latter uses his fragment to grow larger and more powerful as he absorbs more energy. Eventually, the Spider-Men are able to defeat the villains and claim their fragments before heading off to find more. The Amazing Spider-Man finds another fragment at an abandoned
Roxxon Industries quarry, but it is stolen by
Sandman, who uses it to increase his powers and take over the quarry. Spider-Man Noir pursues the
Vulture, Osborn's right-hand man and his
Uncle Ben's killer, for a fragment, which granted the villain teleportation powers. Spider-Man 2099 faces his half-brother
Kron Stone / Scorpion, who has stolen a fragment for a contractor in exchange for reversing his mutation, gaining the ability to create numerous offspring in the process. The Ultimate Spider-Man competes in
Deadpool's reality TV show,
Pain Factor, for a fragment, which the latter eventually uses to clone himself. Meanwhile, Mysterio discovers his fragment granted him magic powers and seeks out the rest. When the Spider-Men return to Madame Web with the fragments they collected, Mysterio attacks, threatening to kill Madame Web if they do not deliver the remaining fragments to him. The Amazing Spider-Man finds his final fragment at a construction yard, but is forced to intervene in
Silver Sable and the
Wild Pack's chase of the
Juggernaut after the latter unknowingly picks it up. Spider-Man Noir faces Osborn for his final fragment, which the latter used to mutate himself into a
goblin-like creature and take over a
carnival. Spider-Man 2099 breaks into
Alchemax, where he finds his final fragment in the hands of
Dr. Serena Patel /
Doctor Octopus, Hobgoblin's creator and Scorpion's contractor. The Ultimate Spider-Man finds his final fragment at the Triskelion, where
S.H.I.E.L.D. unwittingly combined it with
Carnage, giving the creature the ability to resurrect its victims into
zombie-like minions and allowing it to break free and take over the base. After retrieving the final set of fragments, the Spider-Men return to Mysterio, who absorbs the complete Tablet after a brief struggle and becomes a
god. He shatters the borders between realities, seeking to create a new one under his rule. However, this allows Madame Web to summon the four Spider-Men to fight Mysterio together. After defeating Mysterio and separating him from the Tablet, the multiverse is restored and the multiversal Spider-Men return to their native realities while the Amazing Spider-Man takes Mysterio to prison. In a
post-credits scene, Madame Web is visited by
Spider-Ham, who had also hoped to help save the multiverse, but arrived too late. ==Cast==