C.O.W.-Boys •
Marshal Moo Montana (voiced by
Pat Fraley) is the leader of the C.O.W.-Boys and the marshal of Moo Mesa. Courageous and quick on his hooves, Moo "battles the bad guys and makes the West a safer place to graze". Lives by the Code of the West, which he seems to make up as he goes along (as seen in the episode "The Big Cow Wow"). He has a gun that shoots star-badges and rides a horse named "Cyclone". •
Dakota Dude (voiced by
Jim Cummings) is the soft-spoken muscle of Montana's posse, Dakota is calm and level-headed, as he rarely loses his temper, even in near-death experiences (as seen in "Dances with Bulls") and is scared of heights. Dakota agreed to marry Cowlamity Kate in
Wedding Bull Blues to save her father's inheritance. Dakota's horse's name is "Rebel". •
Cowlorado Kid (voiced by
Jeff Bennett) is a
Holstein cattle bull who is the youngest of the group and a self-proclaimed ladies' man with a good singing voice, regardless of his skill with the lasso and guitar; also, Cowlorado isn't a deputy yet. In "Stolen on the River", he tries to prove he's worthy of being a deputy by catching Five Card Cud only to get in trouble and be rescued by Dakota and Moo. Cowlorado's horse's name is "Jezebel".
Supporting •
Lily Bovine (voiced by Charity James) is a
bartender, former
showgirl, and the owner of the local saloon called
The Tumbleweed where the C.O.W.-Boys go to enjoy some
Sarsaparilla. Lily is Moo Montana's love interest. Her best friend is Cowlamity Kate. •
Cody Calf (voiced by Troy Davidson as a kid,
Rob Paulsen as an adult in "Skull Duggery Rides Again") is a calf who idolizes Moo Montana and hopes to be a lawcow himself when he grows up. He is nicknamed "Calf-Pint" by the C.O.W.-Boys, is apparently related to Lily Bovine in some way, and lives with her, though he does not appear to be her son. Like everyone else, he refers to her as "Miss Lily". Although well-meaning, he often gets himself into serious trouble trying to "help" the lawcows, but has been a useful asset on several occasions. •
Cowlamity Kate Cudster (voiced by
Kay Lenz) is a
tomboyish
rancher and operator of the highly profitable Golden Cud Mine. She's as hardworking and hard-riding as any bull, and has enough skill with a lasso to put Cowlorado to shame. She returns Dakota's romantic feelings where she once gave him the hat he wears and nearly married him in "Wedding Bull Blues". In "The Fastest Filly in the West", it is revealed that Cowlamity Kate has a cousin named Cowleen. Her name is a play on that of the famous Wild West heroine
Calamity Jane. She owns a horse named "Tarnation". •
Puma (voiced by
Bill Farmer) is a white-bearded
cougar and resident of Cowtown who is the resident
shoeshiner. •
José "J.R." Rey (voiced by
Michael Horse) is an
Indian bison who occasionally aids Moo, Dakota and Cowlorado if the situation needs it. He tends to ramble about the scientific principles of his inventions which the C.O.W.-Boys don't want to listen to and would be asked to show them how it works. •
Tewah (voiced by Charity James) is an Indian bison in J.R.'s tribe. She is J.R.'s niece and is friends with Cody Calf. •
Buffalo Bull (voiced by
Jeff Bennett) is a bison who works as Cowtown's
blacksmith. In the video game, he is a member of the C.O.W.-Boys. His name is a play on
Buffalo Bill. •
Jack (voiced by
Jim Cummings) is a
rabbit who works as a
telegraph operator at Cowtown. •
Gordon Boredon (voiced by
Jim Cummings) is a
prison warden who runs the prison containing the prisoners that the C.O.W.-Boys apprehend.
Antagonists •
Mayor Oscar Bulloney (voiced by
Michael Greer) is the greedy and corrupt mayor of Cowtown in Moo Mesa and one of the main antagonists of the series. Bulloney rigs elections (as seen in "Stolen on the River") and makes taxes so high that the Masked Bull compares it to stealing. He also serves as Cowtown's crooked Justice of the Peace and Bank President. •
Sheriff Terrorbull (voiced by
Joe Piscopo) is a hunched over red-furred cattle and one of the main antagonists of the series who received this position from Mayor Bulloney. Terrorbull uses his sheriff's badge to conceal his evil intentions. When committing crimes, he disguises himself as the "Masked Bull" where he sports a different posture. Terrorbull was forced to leave Cowtown after losing a bet to Moo in "No Face to Hide", to see who can catch Shock Holiday and became sheriff of the remote town of Lonesome Gulch as Bulloney tells him to put up with it until he can think of a way to get him back into Cowtown. At Lonesome Gulch, Terrorbull continued his unlawful exploits whenever Mayor Bulloney is in need of the Masked Bull. •
Saddle Sore Scorpion (voiced by
Jim Cummings) is a
scorpion and one of the comically inept henchmen of Mayor Bulloney and Sheriff Terrorbull. He is slightly smarter and braver than Boot Hill Buzzard, but only just. His name is taken from the
skin ailment on the rear end as a side effect of horse riding. •
Boot Hill Buzzard (voiced by Danny Mann) is a
buzzard who is one of the comically inept henchmen of Mayor Bulloney and Sheriff Terrorbull. As the more dimwitted of the two, he is often saddled with more than his fair share of the grunt work, especially if the job involves something embarrassing or unappealing. His name is taken from an old west slang term for a
graveyard. •
Barney Finkleberg (voiced by
Tim Curry) is a
con artist who used the alias of Jacques La Beef. The Masked Bull tried various plots to discredit him. After learning the truth, Moo Montana briefly boarded the train Barney was on where he took back the stuff he tried to make off with and advised him never to return to Cowtown. •
Horribull is Sheriff Terrorbull's criminal younger brother whom he breaks out of Sinquitten Federal Prison and has him pose as the Masked Bull in order to protect his identity after Puma saw the Masked Bull without his mask on. •
Sadie Wowcow (voiced by
Michael Greer) is a former show cow who is Lily Bovine's sworn rival. In the past, she always tried to upstage Lily due to jealousy over her popularity. While working for Mayor Bulloney, she tried to get revenge by running Lily out of business in order for Mayor Bulloney to claim an oil well under the Tumbleweed Saloon. •
Skull Duggery (voiced by
Jim Cummings) was originally a mean
miner named Tom Duggery who had staked his claim on Skull Mountain. He had struck silver and hid it in a secret chamber within his mine. After he died, Skull Duggery's
ghost haunted the mine. In his second appearance, Skull Duggery planned his revenge on the C.O.W.-Boys by enlisting some ghosts from a ghost town to help him. •
Dark Fang is an owl ghost who was recruited to assist Skull Duggery in his revenge on the C.O.W.-Boys. •
Evil Eye is a black cat ghost who was recruited to assist Skull Duggery in his revenge on the C.O.W.-Boys. •
Five Card Cud (voiced by
Robby Benson) is a criminal who conspired to take control of the Dixie Trixie riverboat. •
Short Change (voiced by
Michael Gough) is Five Card Cud's
weasel henchman. • The
Gila Hooligans are a gang of
gila monsters who crashed Mayor Bulloney's re-election party. • The
Hole in the Ground Gang are a duo of snakes. Their name is a play on the
Hole in the Wall Gang. •
Bat Blastagun (voiced by
Neil Ross) is a
bat outlaw. He and his gang caused trouble for Miller Glen where they even bested Sheriff T-Bone until they were defeated by Moo Montana. In his second appearance, Bat Blastagun and his gang escaped from prison and use a weaponized version of the comet shard as part of a plot to shrink the C.O.W.-Boys and rob Cowtown. His name is a play on
Bat Masterson. •
Gil A. Monster is a
gila monster who is a part of Bat Blastagun's gang. •
Rawhide is a
rattlesnake member of Bat Blastagun's gang who is often seen being carried by Gil A. Monster. •
Sid Arachnid is a
tarantula who is part of Bat Blastagun's gang. He wields a gun that shoots webs. •
Dr. Wolfgang Wolfenstein is a
wolf hermit/
mad scientist who Bat Blastagun enlisted to weaponize the shard of the comet that created Moo Mesa. •
Shock Holliday (voiced by
Michael Bell) is a
bison outlaw and the leader of his gang who captured all the trains while demanding a ransom from the Railway President. He wields dual lightning bolt shooting electric guns. His name is a play on
Doc Holliday. •
Raw Beans (voiced by
Bill Farmer) is a
red wolf outlaw, member of Shock Holliday's gang, and possibly his second-in-command who wields a gun that shoots beans scattershot-style. His name is a play on
Roy Bean. •
The Boar Brothers are a couple of
wild boar outlaws and members of Shock Holliday's gang. They wield clubs in battle. •
Slick Willy Weasel (voiced by
Jeff Bennett) is a
weasel who is member of Shock Holliday's gang. He drives their carriage. •
Cow Belle (voiced by
Ruth Buzzi) is a female outlaw. She and her three sons used Sidewinder City as a refuge for outlaws everywhere in exchange for a share of their heist. Her name is a play on
Belle Starr. •
Butch Cowsidy (voiced by
Pat Fraley) is Cow Belle's eldest, but shortest son with a broken horn. His name is a play on
Butch Cassidy. •
Lone Grunger is Cow Belle's middle son who dresses in a suit. His name is a play on the
Lone Ranger. •
Sundazed Kid (voiced by
Jeff Bennett) is Cow Belle's youngest, but tallest son who is the strongest of her sons. His name is a play on the
Sundance Kid. •
Longhorn Silver (voiced by
Brad Garrett) is a
longhorn pirate captain with a hook for a left hand who leads his pirate crew in causing trouble on Moo Mesa's waterways. His name is a play on
Long John Silver. • '''Cacklin' Kid''' (voiced by
Rob Paulsen) is a small coyote and known outlaw who the C.O.W.-Boys apprehend while he was being targeted by the Bayin' Bunch, whom he once rode with. • The '''Bayin' Bunch''' are a gang of coyotes who target the Cacklin' Kid who hid their loot in a graveyard and will do anything to get it back from him. •
Scavenger (voiced by
Mark Hamill) is a coyote who sports a
Fu Manchu moustache and the leader of the Bayin' Bunch. •
Buzz is a tan-furred coyote and a member of the Bayin' Bunch. •
Chomp is an overweight coyote and a member o the Bayin' Bunch. •
Billy the Kidder (voiced by
Charlie Adler) is a
goat criminal who targeted the lost treasure of the Concudsadors. His name is a play on
Billy the Kid. •
Lester (voiced by
Charlie Adler) is one of Billy the Kidder's lizard henchmen. •
Kisser is one of Billy the Kidder's lizard henchmen. •
The Great Bovini (voiced by
Dorian Harewood) is a
ringmaster who uses a special stone called the Cowinoor Diamond to mesmerize people. As Boot Hill Buzzard was unaffected by the Cowinoor Diamond while Mayor Bulloney and Saddle Sore were, he and the C.O.W.-Boys had to work together to defeat the Great Bovini and free everyone from the mind-control. • The
Marauders are an all-female gang that pretended to be farmers while stealing a gold mint. •
Barb Wire Babs (voiced by
Kate Mulgrew) is a
cougar and the leader of the Marauders. She uses a metal claw shooting grapnel gun. • The
Cow Twins are two unnamed muscular cows and member of the Marauders. • The
Antelope Lady is an unnamed female antelope and member of the Marauders. •
Rooster Cogsbull (voiced by
Michael Bell) is a wagon master and gold thief who masquerades as a creature known as the "Cowgoyle" (a cow-type
gargoyle). His name is a play on
Rooster Cogburn. •
Big Bucks is a
stag claim-jumper who targeted the recently discovered gold. •
Digalong is a
mole and one of Big Bucks' henchman. •
Stub is a
donkey and one of Big Bucks' henchman. •
Fast Willy is a
dog criminal who plotted to steal Cowleen's horse Tornado as part of a bigger plot to rob the
Pony Express. •
Mules Verne is a
mule who plotted to steal the Dixie Trixie where the Invention Convention is so that he can force the inventors on board to build a giant robot cowboy as part of his plot to take over Moo Mesa. His name is a play on
Jules Verne. ==Episode guide==