•
Henry Gibson as Wilbur, a
pig who was almost killed due to being the
runt of the litter. Over time, however, he grows so much that one would never have known he was once a runt. When he learns of his fate of being slaughtered, he instantly breaks down into tears, until Charlotte tells him that she will do whatever it takes to save him. He is a friendly pig, but also prone to
anxiety. •
Debbie Reynolds as Charlotte A. Cavatica, a
spider who lives on a
web in a corner of Homer's
barn above Wilbur's pigpen. She is very loving and motherly, as well as assertive and good at leading the
barnyard group. She sometimes grows frustrated at Wilbur's anxiety issues. At the end of the film, she dies after laying 514 eggs, but three of them decide to stay with Wilbur. •
Paul Lynde as Templeton, a
care-free,
gluttonous and
egotistical rat who lives at Homer's farm. He helps Charlotte get new ideas for her webs on the condition that he is promised food. At the end of the film, as well as
the sequel, he has four bratty children of his own named Henrietta, Lester, Ralphie, and Junior. •
Agnes Moorehead as the Goose, an unnamed
goose who is the one that encourages Wilbur to speak for the first time. Her seven goslings later hatch, although there were actually eight eggs (one was rotten). In the sequel, she was named Gwen. •
Don Messick as: • Jeffrey, a young, undersized
gosling whom Wilbur befriends shortly after his birth. He initially lives, eats, and sleeps with Wilbur rather than his mother and siblings. • Uncle (impersonating
Candy Candido), a county fair pig who wins first prize. • Lamb •
Herb Vigran as Lurvy, Homer's
farmhand who is the first to notice the messages in Charlotte's webs. •
Pamelyn Ferdin as Fern Arable, John's daughter who convinces him to spare Wilbur's life. •
Martha Scott as Mrs. Arable, Fern's mother who first tells her of what was going to happen to Wilbur. •
Bob Holt as Homer Zuckerman, Mrs. Arable's brother and Fern's uncle. •
John Stephenson as: • John Arable, Fern's father. He was about to "do away" with Wilbur until she intervened. • Parking Officer • Mayor • Bandleader •
Danny Bonaduce as Avery Arable, Fern's older brother. • William B. White as Henry Fussy, a boy of about Fern's age, whom she soon starts spending time with while Wilbur is at the fair. •
Dave Madden as: • The Ram, one of the first animals Wilbur meets at Homer's farm. He is the first to tell Wilbur that it's a pig's fate to be slaughtered and turned into smoked bacon and ham, leaving him to die. • Additional Voices •
Joan Gerber as: • Edith Zuckerman, Homer's wife. She comes up with the idea of giving Wilbur a
buttermilk bath. • Mrs. Fussy, Henry's stern mother who never lets him have fun.
Rex Allen narrates this film. ==Production==