Major League Soccer announced a medical protocol, in consultation with experts, to be used for the tournament to ensure the health and safety of players, coaches, officials, and staff. This included testing for
COVID-19 prior to and throughout the tournament, wearing a face covering or mask, and
social distancing to prevent an outbreak of COVID-19 from occurring. Players and staff who were deemed "high-risk individuals" were not permitted to attend the tournament unless medically cleared. Had anyone tested positive for COVID-19, they were required to
isolate under a strict and detailed protocol to prevent transmission. Prior to travelling to Orlando, all players, coaches, referees, club personnel and league staff were required to complete two
Polymerase Chain Reaction (PCR) tests 24 hours apart and 72 hours before travelling to the ESPN Wide World of Sports Complex. Upon arrival, all individuals were required to take another PCR test and are quarantined until the results of the tests arrive. All individuals who tested positive underwent a clinical assessment by a healthcare provider and moved to an isolation area of the hotel until they were medically cleared. Major League Soccer started producing updates on the testing results of the PCR tests performed in Orlando on June 28. Up to that point, 329 individuals were tested for COVID-19, and two players tested positive, both of whom had just arrived at the facility. Within the next two days, 392 more individuals were tested, of which four of them tested positive. FC Dallas then provided an update on July 1 confirming that six of their players had tested positive for COVID-19, and the rest of their delegation had been quarantined in their hotel rooms pending the results of additional testing. From July 1 to 2, 855 individuals were tested, six of whom were tested positive for COVID-19, four of which were players. From July 3 to 4, two more players tested positive for COVID-19. Between July 7 and 8, Major League Soccer reported that four individuals tested positive for COVID-19, out of 1888 individuals tested. after their opening match was initially postponed. On July 9, 2020,
Nashville SC were also withdrawn from the tournament after nine players of the club tested positive for the virus, after their opening match was initially postponed. On July 12, the match between
Toronto FC and
D.C. United was postponed just minutes prior to kickoff after at least one player was tested positive for COVID-19. The game was rescheduled for the following day on July 13, while the two players who received the positive and inconclusive test were both medically cleared to resume activities. From July 16 onward, the MLS did not record a single positive test of COVID-19 within the delegation staying at the complex. ==Schedule==