T.U.F.F. The Turbo Undercover Fighting Force (T.U.F.F.) headquarters (HQ) is an organization that protects Petropolis from crime. • Dudley Puppy (voiced by
Jerry Trainor) is a white
mixed-breed dog who wears a black t-shirt. • Kitty Katswell (voiced by
Grey DeLisle) is a tan cat who's Dudley Puppy's partner. • The Chief (voiced by
Daran Norris) is a good-natured flea who leads T.U.F.F. HQ. • Keswick (voiced by
Jeff Bennett) is a prairie dog who's a scientist with glasses. Also in T.U.F.F. are various personnel that appear as background characters whether as agents, office workers, and/or scientists. Some of the minor members of T.U.F.F. that have been identified or mentioned include: • Agent Groundhog (voiced by Jeff Bennett) is a T.U.F.F. agent who first made a cameo in "Hot Dog". He resides under an office printer and is the one who tells about the end of winter. • Agent Jumbo (voiced by Grey DeLisle) is an elephant T.U.F.F. agent who has a fear of mice. This results in her jumping up when she sees them and accidentally crushing others. • Agent Moby (voiced by
Maddie Taylor) is a whale T.U.F.F. agent. Like Jumbo, Moby has a tendency to accidentally crush the Chief. • Agent Nutz (voiced by Jeff Bennett) is a squirrel T.U.F.F. agent who has a tendency to blast anything that scares him with his laser gun. • Tammy (voiced by Grey DeLisle) is a rabbit that works as a secretary at T.U.F.F. HQ.
Villains • D.O.O.M. (short for Diabolical Order of Mayhem) is an evil organization that are the main enemies of T.U.F.F. • Verminious Snaptrap (voiced by Maddie Taylor) is an evil rat who is the leader of the Diabolical Order of Mayhem. • Larry (voiced by Jeff Bennett) is a
shrew and Snaptrap's brother-in-law who is often mistreated by him. • Ollie (voiced by Jeff Bennett) is a sensible and smart British possum who is one of Snaptrap's henchmen. • Francisco (voiced by Daran Norris) is a green alligator with red and yellow eyes who is one of Snaptrap's henchmen. • Bad Dog (voiced by Jeff Bennett in the first appearance, Daran Norris in later appearances) is a
pit bull who is one of Snaptrap's henchmen. The character does not appear after the first season. • Leather Teddy (voiced by Eric Bauza) is a leather clad
blue bear and one of Snaptrap's henchmen. He wears an eyepatch despite having a working eye. The character does not appear after the first season. • The Mole (voiced by Maddie Taylor) is a literal mole who is a spy for Snaptrap. The character does not appear after the first season. • The Chameleon (voiced by Daran Norris impersonating
Peter Lorre) is an evil green chameleon who is one of the main antagonists of the series. He wear a molecular transformation suit which allows him to blend in with walls and disguise himself as anything or anyone. • Bird Brain (voiced by
Rob Paulsen) is an evil blue-bottomed booby bird criminal genius who cannot fly naturally. • Zippy (voiced by Grey DeLisle) is Bird Brain's scatterbrained
hummingbird sidekick/partner. • Owl (voiced by Jeff Bennett) is an owl. • Bat (voiced by Daran Norris impersonating
Bela Lugosi) who wears a bat in sunglasses. • Duck (voiced by Jeff Bennett) is a duck whose name causes the rest of Bird Brain's henchmen to actually duck. • Ewe (voiced by Grey DeLisle) is a female sheep. • R.I.T.A. (voiced by Grey DeLisle) is a super-intelligent toaster created by Keswick. • Snowflake and Slush (voiced by
Mary Birdsong and
Dave Boat) are evil rabbit siblings. • Dr. Rabies and Madame Catastrophe (voiced by Jerry Trainor and Grey DeLisle) are two villains who resemble Dudley Puppy and Kitty Katswell with eyepatches and goatees. • Crazy Horse (voiced by Daran Norris) is a horse who uses chainsaws. • Jack Rabbit (voiced by Daran Norris) is Kitty's T.U.F.F. partner. • The Caped Cod (voiced by
Chris Parnell in the first appearance,
Mick Wingert in later appearance) is a crazy cod who thinks he is the ruler of the seven seas. • The Stink Bug (voiced by
Carlos Alazraqui) is Petropolis' worst smelling villain. • Desirah (voiced by
Kari Wahlgren) is a golden jackal genie who grants wishes to masters and mistresses. • Lunch Lady Bug (voiced by
Candi Milo) is a
ladybug who worked as a
lunch lady at Petropolis High School and makes disgusting food that only Dudley likes. • F.L.O.P.P. (short for Fiendish League of Potential Perpetrators) is a criminal organization that would often do minor criminal activities. • Meerkat (voiced by Daran Norris impersonating
Paul Lynde) is a meerkat with a black cowboy hat who is the eccentric leader of F.L.O.P.P. • Wanna-Bee (voiced by Jerry Trainor) is a bee with a high voice who is a member of F.L.O.P.P. • Escape Goat (voiced by Maddie Taylor impersonating
Paul Reubens) is an
escape artist goat who is a member of F.L.O.P.P. • Quacky the Duck (voiced by Maddie Taylor impersonating
Bobcat Goldthwaite) was originally a kid show host seen in "Kid Stuff" until he later went rogue when he heard that his show was getting cancelled. • The Sharing Moose (voiced by Jeff Bennett) is a moose that is the buff and violent best friend of Quacky the Duck. • Katty Katswell (voiced by Grey DeLisle) is Kitty Katswell's evil twin sister.
Recurring characters • Peg Puppy (voiced by
Leslie Carrara-Rudolph) is Dudley's overprotective mother. • Mrs. Katswell (voiced by Grey DeLisle) is Kitty and Katty Katswell's mother. • Wolf Spitzer (voiced by Daran Norris) is a
wolf who is Petropolis local
news reporter for KPET News. • Eric (voiced by Daran Norris) is a buff cat who delivers the water to T.U.F.F. • Bob Barky (voiced by Daran Norris) is a dog game show host who hosts various game shows in Petropolis.. • Phil (voiced by Maddie Taylor) is a bulldog who is Dudley's friend. • Mr. Wong (voiced by Maddie Taylor) is a duck who is the owner of a buffet restaurant. ==Production==