left as
grave goods, exactly as laid out in the Great Tomb at
Gebelein,
Egypt, 2435–2305 BC
Brewing and winemaking Natural fermentation predates human history. Since ancient times, humans have exploited the fermentation process. They likely began fermenting foods unintentionally. To store excess foods, humans placed the items in a container where they were forgotten. Over time, yeast and bacteria started to grow. This led humans to discover fermented foods. The earliest archaeological evidence of fermentation is 13,000-year-old residues of a beer, with the consistency of gruel, found in a cave near
Haifa in Israel. Another early alcoholic drink, made from fruit, rice, and honey, dates from 7000 to 6600 BC, in the
Neolithic Chinese village of
Jiahu, and winemaking dates from ca. 6000 BC, in
Georgia, in the
Caucasus area. Seven-thousand-year-old jars containing the remains of wine, now on display at the University of Pennsylvania, were excavated in the
Zagros Mountains in
Iran. There is strong evidence that people were fermenting alcoholic drinks in
Babylon ca. 3000 BC,
ancient Egypt ca. 3150 BC, pre-Hispanic Mexico ca. 2000 BC,
Discovery of the role of yeast The French chemist
Louis Pasteur founded
zymology, when in 1856 he connected yeast to fermentation. When studying the fermentation of sugar to
alcohol by
yeast, Pasteur concluded that the fermentation was catalyzed by a vital force, called "
ferments", within the yeast cells. The "ferments" were thought to function only within living organisms. Pasteur wrote that "Alcoholic fermentation is an act correlated with the life and organization of the yeast cells, not with the death or putrefaction of the cells."
"Cell-free fermentation" Nevertheless, it was known that yeast extracts can ferment sugar even in the absence of living yeast
cells. While studying this process in 1897, the German chemist and zymologist
Eduard Buchner of
Humboldt University of Berlin, Germany, found that sugar was fermented even when there were no living yeast cells in the mixture, by an enzyme complex secreted by yeast that he termed
zymase. In 1907 he received the
Nobel Prize in Chemistry for his research and discovery of "cell-free fermentation". One year earlier, in 1906,
ethanol fermentation studies led to the early discovery of oxidized
nicotinamide adenine dinucleotide (NAD+). == Uses ==